Protein

Protein accession
W5RAM3 [UniProt]
Representative
7yFAa
Source
UniProt (cluster: phalp2_20990)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MLMTKNQAEKWFDNSLGKQFNPDGWYGFQCYDYANMFFMLATGERLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPNNLSVGNKAKGIIKQATTKKEAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSELGFITNKNDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNQKNPPVPAGYTLDKNNVPYKKEQGNYTVANVKGNNVRDGYSTNSRITGVLSNNTTITYDGAYCINGYRWITYIANSGQRRYIATGEVDKAGNRISSFGKFSAV
Physico‐chemical
properties
protein length:484 AA
molecular weight:53745,8 Da
isoelectric point:9,29
hydropathy:-0,50
Representative Protein Details
Accession
7yFAa
Protein name
7yFAa
Sequence length
308 AA
Molecular weight
34727,87560 Da
Isoelectric point
8,90503
Sequence
MLMTRNQAEKWFDNSLGKQFNPDGWYGFQCYDYANMFFMLATGERLQGLYAYNIPFDNKAKIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESANLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDNPMYFIRLNFPDSISVKDKAKGIIKQATAKKEAVIKPKRIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLRHAGHEVALYGGSSQSQDMYQDTAYGVNVGNKKDYGLYWVKSQGYDIVLEIHLDAAGESASGGHVIISSQFNADTIDKKVYKMLLKITWDK
Other Proteins in cluster: phalp2_20990
Total (incl. this protein): 82 Avg length: 428,8 Avg pI: 9,14
Protein ID Length (AA) pI
7yFAa 308 8,90503
73a4P 176 8,80704
75Ban 334 7,74155
78B87 220 9,34200
79Nar 183 9,28475
7Rfws 257 9,36179
7Ribo 188 9,28456
7a6Vp 334 6,45187
7aH2t 183 9,28475
7aN38 327 9,03365
7bAZA 435 9,16400
7c3Bd 189 9,46107
7duLE 216 9,18644
7f4NS 299 7,09171
7kRuA 265 9,34890
7kroY 304 8,60790
7l17s 304 8,91438
8tcB1 255 9,53844
A7TWE3 484 9,28617
A0A068A229 484 9,18915
A0ZS57 484 9,27934
A0A6F8Z5Y7 141 7,64083
A0A411BM53 484 9,29313
A0A2K9VBR6 484 9,32730
A0A345AQC9 484 9,28617
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_22245
7e47k
 317 62,1% 301 7.188E-141
2 phalp2_10799
3WA57
 47 29,3% 218 5.253E-12
3 phalp2_35363
7xThI
 2 27,7% 288 4.009E-11

Domains

Domains [InterPro]
CHAP
Ami3
Representative sequence (used for alignment): 7yFAa (308 AA)
Member sequence: W5RAM3 (484 AA)
1 308 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01520, PF05257

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage StauST398-4
[NCBI] 		1308896 Biseptimavirus > Biseptimavirus StauST3984
Host Staphylococcus aureus
[NCBI] 		1280 Firmicutes > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
KC595278 [NCBI]
CDS location
range 38678 -> 40132
strand +
CDS
ATGTTAATGACAAAAAATCAAGCAGAAAAATGGTTCGACAATTCACTAGGGAAACAATTTAATCCAGATGGTTGGTATGGATTTCAGTGTTATGATTATGCCAATATGTTCTTTATGTTAGCGACAGGCGAAAGGCTGCAAGGTTTATATGCTTATAACATCCCGTTTGATAATAAAGCGAAGATTGAAAAATATGGTCAAATAATTAAAAACTATGACAGCTTTTTGCCGCAAAAGTTGGATATTGTCGTCTTCCCATCGAAGTATGGTGGTGGAGCTGGACACGTTGAAATTGTTGAGAGCGCAAATTTAAATACTTTCACATCATTTGGTCAAAACTGGAACGGTAAAGGTTGGACTAATGGCGTTGCGCAACCTGGTTGGGGTCCTGAAACTGTGACAAGACATGTTCATTATTATGACAATCCAATGTATTTTATTAGGTTAAACTTCCCTAACAACTTAAGCGTTGGCAATAAAGCTAAAGGTATTATTAAGCAAGCGACTACAAAAAAAGAGGCAGTAATTAAACCTAAAAAAATTATGCTTGTAGCCGGTCATGGTTATAACGATCCTGGAGCAGTAGGAAACGGAACAAACGAACGCGATTTTATACGTAAATATATAACGCCTAATATCGCTAAGTATTTAAGACATGCAGGACATGAAGTTGCATTATACGGTGGCTCAAGTCAATCACAAGATATGTATCAAGATACTGCATACGGTGTTAATGTAGGCAATAAAAAAGATTATGGCTTATATTGGGTTAAATCACAGGGGTATGACATTGTTCTAGAAATACATTTAGACGCAGCAGGAGAAAGCGCAAGTGGTGGGCATGTTATTATCTCAAGTCAATTCAATGCAGATACTATTGATAAAAGTATACAAGATGTTATTAAAAATAACTTAGGACAAATAAGAGGTGTGACACCTCGTAATGATTTACTAAATGTTAATGTATCAGCAGAAATAAATATAAATTATCGTTTATCTGAATTAGGTTTTATTACTAATAAAAATGATATGGATTGGATTAAGAAAAACTATGACTTGTATTCTAAATTAATAGCCGGTGCGATTCATGGTAAGCCTATAGGTGGTTTGGTAGCTGGTAATGTTAAAACATCAGCTAAAAACCAAAAAAATCCACCAGTGCCAGCAGGTTATACACTCGATAAGAATAATGTCCCTTATAAAAAAGAACAAGGCAATTACACAGTAGCTAATGTTAAAGGTAATAATGTAAGAGACGGTTATTCAACTAATTCAAGAATTACAGGGGTATTATCCAACAACACAACAATTACGTATGACGGTGCATATTGTATTAATGGTTATAGATGGATTACTTATATTGCTAATAGTGGACAACGTCGTTATATAGCGACAGGAGAGGTAGACAAGGCAGGTAATAGAATAAGTAGTTTTGGTAAGTTTAGTGCAGTTTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

PDB ID
upi0002432ca1_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7yFAa) rather than this protein.
PDB ID
7yFAa
Method AlphaFoldv2
Resolution 89.01
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50