Protein

Protein accession
Q8SDS7 [UniProt]
Representative
7B0ac
Source
UniProt (cluster: phalp2_30749)
Protein name
Endolysin LytA
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSIIMEVATMQAKLTKNEFIEWLKTSEGKQFNVDLWYGFQCFDYANAGWKVLFGLLLKGLGAKDIPFANNFDGLATVYQNTPDFLAQPGDMVVFGSNYGAGYGHVAWVIEATLDYIIVYEQNWLGGGWTDGIEQPGWGWEKVTRRQHAYDFPMWFIRPNFKSETAPRSVQSPTQAPKKETAKPQPKAVELKIIKDVVKGYDLPKRGSNPKGIVIHNDAGSKGATAEAYRNGLVNAPLSRLEAGIAHSYVSGNTVWQALDESQVGWHTANQIGNKYYYGIEVCQSMGADNATFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKYGTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTEVMLQDGHVWVGYTWEGQRYYLPIRTWNGSAPPNQILGDLWGEIS
Physico‐chemical
properties
protein length:490 AA
molecular weight:55005,6 Da
isoelectric point:8,48
hydropathy:-0,43
Representative Protein Details
Accession
7B0ac
Protein name
7B0ac
Sequence length
210 AA
Molecular weight
23904,72030 Da
Isoelectric point
8,75901
Sequence
MCQSMGADNSTFLKNEQATFQECARLLKKWGLPANRNTIRLHNEFTSTSCPHRSSVLHTGFDPVTRGLLPEDKRLQLKDYFIKQIRAYMDGKIPVATVSNESSASSNTVKPVASAWKRNKYGTYYMEESARFTNGNQPITVRKVGPFLSCPVGYQFQPGGYCDYTSVLLQDNHVWLEYEWQGNFYYIPIRSWDGTPPPNQIVGELWGTIS
Other Proteins in cluster: phalp2_30749
Total (incl. this protein): 146 Avg length: 312,5 Avg pI: 8,70
Protein ID Length (AA) pI
7B0ac 210 8,75901
1RhwD 168 9,70947
5tFp 249 9,51774
75B9L 295 8,82097
77ve9 237 8,51971
7aTPL 270 9,62811
7auDn 237 8,51120
82Zok 270 9,27205
8LHis 209 9,49382
8fsmJ 249 9,56629
Db4c 149 9,91480
oPCI 257 9,63843
ym82 179 10,01112
Q4ZA77 210 8,75901
A0A7T1JPU3 481 8,73252
S4SVK6 481 8,73252
A0A3G2YSI2 249 6,41220
A0A6M2Z157 249 7,58951
A0A7L8ZIX6 249 6,57692
Q4ZCC3 481 9,16645
A0A410T4J4 490 8,61409
B8QIR1 481 8,73787
Q4ZC44 482 9,20501
Q4ZAU2 481 8,72723
A0A173G9X3 250 7,57177
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_7790
7qCWn
 51 38,7% 214 1.085E-57
2 phalp2_15359
1El58
 3 28,2% 195 3.560E-20
3 phalp2_20292
2aQ1n
 8 23,9% 213 3.528E-12
4 phalp2_24730
6BIOG
 3 19,7% 203 8.441E-06
5 phalp2_28103
7vQh7
 9 20,5% 214 2.136E-04

Domains

Domains [InterPro]
Unannotated
SH3_5
Representative sequence (used for alignment): 7B0ac (210 AA)
Member sequence: Q8SDS7 (490 AA)
1 210 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF08460

Taxonomy

  Name Taxonomy ID Lineage
Phage Staphylococcus phage phi11 (Bacteriophage phi-11)
[NCBI] 		2681609 Dubowvirus > Dubowvirus dv11
Host Staphylococcus aureus
[NCBI] 		1280 Firmicutes > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus >

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
AF424781 [NCBI]
CDS location
range 40920 -> 42365
strand +
CDS
ATGCAAGCAAAATTAACTAAAAATGAGTTTATAGAGTGGTTGAAAACTTCTGAGGGAAAACAATTCAATGTGGACTTATGGTATGGATTTCAATGCTTTGATTATGCCAATGCTGGTTGGAAAGTTTTGTTTGGATTACTTCTAAAAGGTTTAGGTGCAAAAGATATTCCGTTCGCTAACAACTTCGACGGATTAGCTACTGTATACCAAAATACACCGGACTTCTTAGCACAACCTGGCGACATGGTGGTATTCGGTAGCAACTACGGTGCTGGATATGGTCACGTTGCATGGGTAATTGAAGCAACTTTAGATTACATCATTGTATATGAGCAGAATTGGCTAGGCGGTGGCTGGACTGACGGAATCGAACAACCCGGCTGGGGTTGGGAAAAAGTTACAAGACGACAACATGCTTATGATTTCCCTATGTGGTTTATCCGTCCGAATTTTAAAAGTGAGACAGCGCCACGATCAGTTCAATCTCCTACACAAGCACCTAAAAAAGAAACAGCTAAGCCACAACCTAAAGCAGTAGAACTTAAAATCATCAAAGATGTGGTTAAAGGTTATGACCTACCTAAGCGTGGTAGTAACCCTAAAGGTATAGTTATACACAACGACGCAGGGAGCAAAGGGGCGACTGCTGAAGCATATCGTAACGGATTAGTAAATGCACCTTTATCAAGATTAGAAGCGGGCATTGCGCATAGTTACGTATCAGGCAACACAGTTTGGCAAGCCTTAGATGAATCACAAGTAGGTTGGCATACCGCTAATCAAATAGGTAATAAATATTATTACGGTATTGAAGTATGTCAATCAATGGGCGCAGATAACGCGACATTCTTAAAAAATGAACAGGCAACTTTCCAAGAATGCGCTAGATTGTTGAAAAAATGGGGATTACCAGCAAACAGAAATACAATCAGATTGCACAATGAATTTACTTCAACATCATGCCCTCATAGAAGTTCGGTTTTACACACTGGTTTTGACCCAGTAACTCGCGGTCTATTGCCAGAAGACAAGCGGTTGCAACTTAAAGACTACTTTATCAAGCAGATTAGGGCGTACATGGATGGTAAAATACCGGTTGCCACTGTCTCTAATGAGTCAAGCGCTTCAAGTAATACAGTTAAACCAGTTGCAAGTGCATGGAAACGTAATAAATATGGTACTTACTACATGGAAGAAAGTGCTAGATTCACAAACGGCAATCAACCAATCACAGTAAGAAAAGTGGGGCCATTCTTATCTTGTCCAGTGGGTTATCAGTTCCAACCTGGTGGGTATTGTGATTATACAGAAGTGATGTTACAAGATGGTCATGTTTGGGTAGGATATACATGGGAGGGGCAACGTTATTACTTGCCTATTAGAACATGGAATGGTTCTGCCCCACCTAATCAGATATTAGGTGACTTATGGGGAGAAATCAGTTAG

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0006508 proteolysis biological process None (UniProt)
GO:0008233 peptidase activity molecular function None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0046872 metal ion binding molecular function None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.4.22.- None None N/A UniProt
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. experimental evidence used in manual assertion
ECO:ECO:0000269 		PubMed:10336488

Tertiary structure

PDB ID
P79668
Method SMR
Resolution –
Chain position –
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
Q8SDS7
Method AlphaFoldv2
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
PDB ID
upi000009b2cc_model
Method AlphaFold3 (non-commercial)
Resolution -
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50
The structures below correspond to the cluster representative (7B0ac) rather than this protein.
PDB ID
7B0ac
Method AlphaFoldv2
Resolution 81.37
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50