Protein

Protein accession
A0A5Q2WFK5 [UniProt]
Representative
7wVIM
Source
UniProt (cluster: phalp2_17757)
Protein name
N-acetylmuramoyl-L-alanine amidase
Lysin probability
100%
PhaLP type
endolysin
Probability: 99% (predicted by ML model)
Protein sequence
MSFVQKNATPLDTEENIARRVHAVSIKRGQKNPELASVITLMGIRQESDFWCPWNRKDPSSEKYDHDSESDDGRSVGYLQQQNGRAGEVLPPGDRDNWWGSMAQRMGLESAVDTFQDRMSEDYQKVSEPWGANAIIQRVQGSGVPDAYAKHWDYCWSLLRRALATSPTPLPPLVPSPVKPAPTQPPAPVIQPNPGHRGDPLYLPELLRAFGVSVSTYTDQEGIPWDQRGHGDFGVIDYVVWHHTGSVNETDVGIAHHPALGLAATMLVHEDGHVVLTGSGIAWHAGDGIWPGVARGAMNQRSIGIECAYSWGPQELHNPWPPEQLQAMINIGGAISWYLQGTLPPDHQIAHKEWAGAENPLGINAQGKPDPANLDMDWFRGEIAKRAAAGPSGGGSTTEEGFLMGLSQEDQIAIRDKILGYPSLDHGWPSRSIFARGPGKVDDTVGILLFTDGNAFNLNVILGAMAGFEPFAEDVRRVAEHGPAEGTFAAEHYPEDAKAIAQAFVPLIGTLPQLMALATGPRGDLTGPAPAKKAAPKKRAKKAAPTGEVTE
Physico‐chemical
properties
protein length:551 AA
molecular weight:59649,2 Da
isoelectric point:5,40
hydropathy:-0,43
Representative Protein Details
Accession
7wVIM
Protein name
7wVIM
Sequence length
464 AA
Molecular weight
50839,29520 Da
Isoelectric point
5,92037
Sequence
MVTKDQVAQLIVGEAKSRNHTRDECLAEMSALYQESQWDETVWDPTHTTYGVAQQDGSYPNRFQGAAAQIKAFFDKLDVKRASPGRGDIWLNICWLQQAPNWLSAQYWYDNGRRAYLAEIKSRVATVTPYLERYWPATGTPTIPVDTAPPVGDPVWLPDVLRAAGLRCDIYPGAMDRGHGDFGRIWGVVCHHTGSFGETPRGIAEHPELGLASQLYLSRDGVFTLCGVGIAWHAGAGSWPGIPTNNANQVTIGIEAANDGGGTPGKPHRTGWSDAQYNAYVTGVAAILNKLGQPADHAIGHKEWAGAAQGKWDPGAIDMNIFRADVAREQARLKGAASTDPIEELLMMELESTSPFATPGEPKIPAEVMLRNADAAGHRALVLDDAEDGDQDAIFRFVRAARGHDSRYTDAPSVAKWRRRLNRFIRRTAVDAASGDTTAVGILRHMQDKYPDVLQDFINTRKGN
Other Proteins in cluster: phalp2_17757
Total (incl. this protein): 110 Avg length: 435,2 Avg pI: 6,17
Protein ID Length (AA) pI
7wVIM 464 5,92037
3lLVF 407 5,20409
6Ugxv 424 4,52236
6Vwoo 424 4,48627
7AK6J 414 6,04019
7aY3y 406 5,95709
7iKFq 416 5,65562
7r406 392 5,71450
7ubx8 386 5,04221
7udcz 390 5,05955
8dQhs 408 5,38933
A0A4D6E276 354 8,76559
A0A455LM17 510 5,77498
A0A386KB78 528 5,68301
G8IAJ2 476 5,87729
G8I9G3 577 5,33737
A0A3Q9R8M1 366 6,76346
A0A1B3AZZ3 366 6,59755
A0A162E1U7 370 6,63813
A0A2Z4Q823 381 6,11942
A0A2P1A0T5 366 6,95672
A0A159B6C4 366 6,56680
A0A2K9VH40 366 6,97906
A0A2Z4Q8W9 381 6,14489
A0A346FCL4 344 6,75704
Similar Clusters (pHMM search)
# Cluster # Members Identity (%) Alignment Length E-value
1 phalp2_40628
7RbAk
 146 46,5% 329 1.367E-98

Domains

Domains [InterPro]
Unannotated
Ami2
Unannotated
Representative sequence (used for alignment): 7wVIM (464 AA)
Member sequence: A0A5Q2WFK5 (551 AA)
1 464 AA (representative)
Domain positions follow the representative sequence above; the member sequence bar is scaled to the same axis.
Legend: EAD CBD Linker Disordered Unannotated
Pfam accessions: PF01510

Taxonomy

  Name Taxonomy ID Lineage
Phage Mycobacterium phage Quesadilla
[NCBI] 		2664226 Quesadillavirus > Quesadillavirus quesadilla
Host No host information

Coding sequence (CDS)

Coding sequence (CDS)
CDS Source ID
[NCBI]
CDS Source
MN617843 [NCBI]
CDS location
range 42178 -> 43833
strand +
CDS
GTGAGCTTCGTGCAGAAGAACGCGACCCCTCTGGACACCGAGGAGAACATCGCTCGGCGGGTCCACGCGGTCAGCATCAAGCGCGGCCAGAAGAACCCCGAGCTGGCCTCGGTCATCACCCTGATGGGCATCCGCCAGGAGAGCGACTTCTGGTGCCCTTGGAACCGCAAAGACCCCAGCTCGGAGAAGTACGACCACGACTCCGAGAGCGATGACGGCCGCAGCGTCGGCTATCTCCAGCAGCAGAACGGCCGGGCGGGCGAGGTGCTCCCGCCCGGCGACCGCGACAACTGGTGGGGGTCGATGGCCCAGCGCATGGGCCTGGAGAGCGCCGTCGATACGTTCCAAGATCGGATGTCGGAGGATTACCAGAAGGTCTCCGAGCCGTGGGGCGCGAACGCCATCATCCAGCGCGTCCAGGGCAGCGGCGTTCCCGACGCCTACGCCAAGCACTGGGACTACTGCTGGAGCCTGCTCCGGCGCGCCCTGGCCACCAGCCCGACGCCGCTGCCGCCCCTGGTGCCCTCGCCGGTCAAGCCAGCGCCGACTCAGCCGCCCGCCCCGGTCATCCAGCCCAACCCCGGCCACCGCGGCGATCCGCTCTACCTGCCCGAGCTGCTCCGCGCGTTCGGCGTGAGCGTGAGCACCTACACCGACCAGGAGGGCATCCCCTGGGATCAGCGCGGGCACGGCGACTTCGGGGTCATCGACTATGTGGTCTGGCACCACACCGGCTCGGTCAACGAGACCGACGTGGGCATCGCCCATCACCCTGCGCTGGGCCTGGCCGCGACGATGCTCGTCCATGAGGACGGCCATGTCGTGCTCACCGGCTCGGGCATCGCCTGGCACGCGGGCGACGGCATCTGGCCGGGCGTGGCGCGCGGCGCGATGAACCAACGCTCCATCGGCATCGAGTGCGCCTACAGCTGGGGTCCGCAGGAGCTTCACAACCCCTGGCCACCCGAGCAGCTCCAGGCCATGATCAACATCGGCGGGGCGATCTCCTGGTATCTCCAGGGCACGCTCCCGCCCGACCACCAGATTGCCCACAAGGAGTGGGCCGGGGCCGAGAACCCGCTGGGCATCAACGCCCAGGGCAAGCCAGACCCCGCCAACCTCGACATGGATTGGTTCCGTGGCGAGATCGCCAAGCGTGCCGCAGCCGGGCCATCCGGCGGCGGCTCCACAACCGAGGAGGGGTTCCTGATGGGCCTATCGCAAGAGGACCAGATCGCCATCCGCGACAAGATTCTGGGCTACCCGAGCCTGGACCACGGCTGGCCGAGCCGGTCGATCTTCGCGCGCGGGCCGGGCAAGGTGGACGACACCGTGGGCATCCTGCTGTTCACCGATGGCAATGCGTTCAACCTCAACGTCATCCTGGGCGCGATGGCCGGGTTCGAGCCGTTCGCCGAGGACGTGCGCCGGGTCGCCGAGCATGGCCCGGCCGAGGGCACGTTCGCCGCCGAGCACTACCCCGAGGACGCCAAGGCCATCGCCCAGGCGTTCGTGCCGCTGATCGGGACGCTGCCCCAGCTGATGGCGCTCGCCACCGGCCCTCGCGGCGATCTCACCGGCCCGGCTCCGGCGAAGAAGGCCGCGCCCAAGAAGCGCGCCAAGAAGGCCGCGCCGACCGGCGAGGTGACTGAGTGA

Gene Ontology

Description Category Evidence (source)
GO:0001897 symbiont-mediated cytolysis of host cell biological process None (UniProt)
GO:0008745 N-acetylmuramoyl-L-alanine amidase activity molecular function None (UniProt)
GO:0009253 peptidoglycan catabolic process biological process None (UniProt)
GO:0009254 peptidoglycan turnover biological process None (UniProt)
GO:0042742 defense response to bacterium biological process None (UniProt)
GO:0071555 cell wall organization biological process None (UniProt)

Enzymatic activity

EC Number Entry Name Reaction Catalyzed Classification Evidence Source
3.5.1.28 None Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides. match to sequence model evidence used in automatic assertion
ECO:ECO:0000256 		ARBA:ARBA00001561

Tertiary structure

No tertiary structures available for this protein.

The structures below correspond to the cluster representative (7wVIM) rather than this protein.
PDB ID
7wVIM
Method AlphaFoldv2
Resolution 82.14
Chain position -
Model Confidence
Very high
pLDDT > 90
High
90 > pLDDT > 70
Low
70 > pLDDT > 50
Very low
pLDDT < 50